thr77 for Dummies
thr77 for Dummies
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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene is observed in several tumors, which leads to chromosome instability, and therefore might add to tumorigenesis. This protein was observed to associate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in cell-cycle controlled histone gene expression and performs a crucial role in endorsing cell-cycle development from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are described. Two supplemental splice variants have been reported but in-depth nucleotide sequence data isn't still obtainable.
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Blocking peptides are peptides that bind especially into the target antibody and block antibody binding. These peptide commonly contains the epitope acknowledged because of the antibody.
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This guidebook outlines additional possible optimization of mobile and immunoassay parameters to be sure the best possible results are...
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This guideline outlines even further probable optimization of mobile and immunoassay parameters to make sure the best possible results are...
Antibodies bound to the blocking peptide no longer bind towards the epitope around the target protein. By comparing the staining from the blocked antibody versus the antibody alone, you can see which staining is particular.
Target Information Cyclin E belongs for the very conserved cyclin household, whose users are characterised by a dramatic periodicity in protein abundance with the cell cycle. Cyclins function as regulators of CDK kinases. Unique cyclins exhibit distinct expression and degradation patterns which lead into the temporal coordination of each and every mitotic celebration. Cyclin E forms a fancy with and functions as a regulatory subunit of CDK2, check here whose activity is necessary for cell cycle G1/S transition. This protein accumulates at the G1-S section boundary and is particularly degraded as cells progress via S section.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by both of those antibodies...
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